Peptone and yeast extract were required for growth [1].

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Pyrosequencing reads were assembled using the Newbler assembler (Roche).

The initial Newbler assembly consisting of 93 contigs in one scaffold was converted into a phrap [40] assembly by making fake reads from the consensus, to collect the read pairs in the 454 paired end library.

The species epithet was derived from the Neo-Latin adjective ‘limnaeae’, living in the water, referring to the microbial mats in Lake Fryxell where the organism was first isolated [1].

Pub Med records do not indicate any follow-up research with strain R-8282 was compared using NCBI BLAST [3,4] under default settings (e.g., considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [5] and the relative frequencies of taxa and keywords (reduced to their stem [6]) were determined, weighted by BLAST scores.

DNA was isolated from 0.5-1 g of cell paste using Master Pure Gram Positive DNA Purification kit (Epicentre MGP04100) following the standard protocol as recommended by the manufacturer with modification st/DL as described by Wu The genome was sequenced using a combination of Illumina and 454 sequencing platforms.

All general aspects of library construction and sequencing can be found at the JGI website [39].

Illumina GAii sequencing data (1,096.5Mb) was assembled with Velvet [41] and the consensus sequences were shredded into 1.5 kb overlapped fake reads and assembled together with the 454 data.

The 454 draft assembly was based on 178.7 Mb 454 draft data and all of the 454 paired end data.

The sequences of the two 16S r RNA gene copies in the genome differ from each other by up to eleven nucleotides, and differ by up to eight nucleotides from the previously published 16S r RNA sequence (AJ440991), which contains seven ambiguous base calls. The tree was inferred from 1,366 aligned characters [7,8] of the 16S r RNA gene sequence under the maximum likelihood (ML) criterion [9].